This kit contains primers to specifically PCR amplify a region of the cyp51 sterol 14 demethylase DNA from Candida albicans and a molecular beacon to detect the mutation leading to fluconazole resistance in the DNA. The kit is very simple to use and involves adding an equal volume of pre-made reaction mixture to your DNA sample.

The resulting mixture is then sealed and loaded into a real-time PCR machine. These are essentially the same as normal PCR machines but have the ability to monitor acculmulation of fluorescent dye in the PCR tube during the reaction

 

RT-PCR machines

 

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