Ecological Archives E089-060-A1

Jay T. Lennon and Kathryn L. Cottingham. 2008. Microbial productivity in variable resource environments. Ecology 89:1001–1014.

Appendix A. Summary of measurements taken from the mesocosms in the field experiment for each treatment, summaries for the repeated-measures analysis of variance (RM-ANOVA) for each response variable in the field experiment, and summaries for the two-way analysis of variance (ANOVA) for cumulative bacterial productivity (BP) and cumulative bacterial respiration (BR) in the field experiment .

TABLE A1. Summary of measurements taken from the mesocosms in the field experiment for each treatment. Dissolved organic carbon (DOC), bacterial productivity (BP), bacterial growth efficiency (BGE), and dissolved oxygen (O2) were measured daily and are expressed as averages across time and replicates within a treatment. Temperature (Temp) and pH were measured on three occasions and are expressed as averages across time and replicates within a treatment. Measurements of chlorophyll a (Chl a), total nitrogen (TN), and total phosphorus (TP), and crustacean zooplankton abundance (Zoop) were obtained on the last day of the experiment. All data represent the mean ± SEM.

Response variable

Control

Low Quality DOC

High Quality DOC

Press

Pulse

Press

Pulse

DOC
(mg/L)

2.4 ± 0.02

3.9 ± 0.32

5.2 ± 0.379

4.1 ± 0.33

5.3 ± 0.39

BP
(mg L-1 d-1)

0.77 ± 0.184

1.44 ± 0.282

4.02 ± 2.135

1.70 ± 0.438

2.59 ±1.127

BR
(mg L-1 d-1)

2.15 ± 0.831

4.43 ± 0.704

5.49 ± 0.734

3.69 ± 0.601

4.43 ± 0.510

BGE

0.30 ± 0.055

0.26 ± 0.040

0.29 ± 0.053

0.30 ± 0.055

0.28 ± 0.048

O2
(mg/L)

6.98 ± 0.031

7.12 ± 0.058

6.88 ± 0.076

6.75 ± 0.094

6.83 ± 0.039

Chl a (mg/L)

7.69 ± 0.078

19.5 ± 1.68

18.9 ± 2.36

33.7 ± 1.57

28.7 ± 2.86

TN
(mg/L)

746 ± 73.7

827 ± 39.2

968 ± 82.8

782 ± 35.1

793 ± 53.9

TP
(mg/L)

6.3 ± 1.32

28.1 ±1.29

32.4 ± 3.09

38.0 ±  2.45

39.5 ± 3.59

Zoop
(#/L)

23.1 ± 3.04

20.6 ±  2.54

29.4 ± 4.27

21.2 ± 3.87

32.6 ±  4.28

Temp
(°C)

20.7 ± 0.35

20.7 ± 0.32

20.7 ± 0.32

20.7 ± 0.32

20.7 ± 0.32

pH

7.2 ± 0.07

7.0 ± 0.08

7.0 ± 0.04

6.9 ± 0.08

7.2 ± 0.11


TABLE A2. Summaries for the repeated-measures analysis of variance (RM-ANOVA) for each response variable in the field experiment.

Response: DOC

covariance structure: compound symmetry

Effect

Num df

Den df

F value

P

time

11

131

1919.17

<0.0001

quality

1

13

6.27

0.0263

schedule

1

13

666.23

<0.0001

time × quality

11

131

1.95

0.0386

time × schedule

11

131

595.9

<0.0001

time × quality × schedule

12

131

2.06

0.0239

         

Response: BP

covariance structure: unstructured

Effect

Num df

Den df

F value

P

time

11

13

2519.41

<0.0001

quality

1

13

2

0.1812

schedule

1

13

20.56

0.0006

time × quality

11

13

368.42

<0.0001

time × schedule

11

13

999.9

<0.0001

time × quality × schedule

12

13

6686.35

<0.0001

         

Response: BR

covariance structure: compound symmetry

Effect

Num df

Den df

F value

P

time

11

131

5.57

<0.0001

quality

1

13

3.65

0.0782

schedule

1

13

1.99

0.182

quality × time

11

131

0.91

0.53

schedule × time

11

131

2.28

0.014

quality × schedule × time

12

131

1.55

0.1159

         

Response: BGE

covariance structure: unstructured

Effect

Num df

Den df

F value

P

time

11

117

6.13

<0.0001

quality

1

13

0.04

0.8367

schedule

1

13

0.02

0.883

quality × time

11

117

0.49

0.9065

schedule × time

11

117

0.32

0.9803

quality × schedule × time

12

117

0.78

0.666


TABLE A3. Summaries for the two-way analysis of variance (ANOVA) for cumulative bacterial productivity (BP) and cumulative bacterial respiration (BR) in the field experiment.

Cumulative BP, two-way ANOVA

Effect

Num df

Den df

SS

F value

P

Quality

1

1

178.6

2.93

0.1127

Schedule

1

1

1436.2

23.56

0.0004

Quality × Schedule

1

1

331.7

5.44

0.0379

           

Cumulative BR, two-way ANOVA

Effect

Num DF

Den DF

SS

F value

P

Quality

1

1

282.9

2.78

0.1212

Schedule

1

1

440.1

4.33

0.0596

Quality × Schedule

1

1

7.7

0.08

0.7882



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