Appendix A. Figures showing a rotating, three-dimensional fragrance space of Euglossa spp. on Barro Colorado Island, Panama, and a full version of Fig. 4.
|FIG. A1. Rotating, three-dimensional fragrance space of Euglossa spp. on BCI. The Multidimensional Scaling (MDS) plot is based on the same data and analysis as the two-dimensional representation in Fig. 2a, but allows one additional dimension and thus accommodates more of the variation in the underlying similarity matrix (Stress = 0.15).|
FIG. A2. An undivided version of Fig. 4: Presence of fragrance compounds in hind leg extracts of 15 sympatric species of Euglossa grouped by their phylogentic relationships. The numbers of individual males analyzed per species are given in parentheses, and shades of gray indicate how much a compound contributed on average to the total peak area in a given species. Compounds are categorized as major (black, >5 % of total peak area), minor (dark gray, 1–5%) and trace (light gray, <1%) components. The numerical data are given in Appendix B. Only the 80 most abundant compounds (across species) are shown, ranked from top to bottom by their retention time on a DB-5 non-polar capillary column (30 m; 60 to 300°C at 3°C/min). The 10 most abundant mass fragments (m/z) are given for unknown compounds. The cladogram corresponds to a 50% majority-rule consensus obtained form a Bayesian tree search; posterior probabilities are shown beside branches (along with bootstrap values of a maximum-likelihood analysis).