Ecological Archives E094-200-A2

Li-Wan Chang, David Zelený, Ching-Feng Li, Shau-Ting Chiu, Chang-Fu Hsieh. 2013. Better environmental data may reverse conclusions about niche- and dispersal-based processes in community assembly. Ecology 94:2145–2151. http://dx.doi.org/10.1890/12-2053.1

Appendix B. Details of soil sample analyses.

In each of the 625 grid cells, three random samples were collected from 0-10 cm depth and mixed together. Soil samples were air-dried and passed through a 2-mm sieve for the following laboratory analyses. The gravimetric soil water content was determined by weight loss at 105°C for 24h (Gardner 1986). The sieved soil samples were subsequently analyzed for total C and N, C/N ratio, pH, extractable K, Ca, Mg, Fe, Mn, Cu, Zn, and P, and soil texture. Total soil C and N were determined by a dry combustion method (Nelson and Sommers 1982) using a Fisons NA-1500 analyzer. Soil pH was measured in 1M KCl solution (soil: solution ratio of 1:2.5 (McLean 1982). Extractable K, Ca, Mg, Fe, Mn, Cu, Zn, and P were measures by the Mehlich-3 solution method (Helmke and Sparks 1996). For soil texture, the Bouyoucos Hydrometer method was used for determining the soil particle size (Gee and Bauder, 1986); for each sample, we obtained percentage of sand, silt, and clay. All analyses were conducted in the Taiwan Forestry Institute soil research laboratory.

 

Literature cited

Gardner, W. H., 1986. Water content. Pages 493–544 in A. Klute, editor. Methods of soil analysis, Part 1. 2nd. Agron. Monogr. 9. ASA and SSSA, Madison, Wisconsin, USA.

Gee, G. W., and J. W. Bauder. 1986. Particle-size analysis: core method. Pages 383–411 in A. Klute, editor. Methods of soil analysis. Part 1. 2nd ed. Agron. Monogr. 9. ASA and SSSA, Madison, Wisconsin, USA.

Helmke, P. A., and D. L. Sparks. 1996. Method of Soil Analyses. Pages 551–601 in D.L. Sparks, editor. Chemical Methods. Part 3. ASA and SSSA, Madison, Wisconsin, USA.

McLean, E. O. 1982. Soil pH and lime requirement. Pages 199–224 in A.L. Page, editor. Methods of soil analysis, Part 2. Chemical and microbioloigical properties. 2nd ed. Agron. Monogr. 9. ASA and SSSA, Madison, Wisconsin, USA.

Nelson, D. W., and L. E. Sommers. Total carbon, organic carbon and organic matter. Pages 539–580 in A.L. Page, editor. Methods of soil analysis, Part 2. Chemical and microbiological properties. Second ed. Agron. Monogr. 9. ASA and SSSA, Madison, Wisconsin, USA.


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