Ecological Archives E094-231-A1

Thomas W. Crowther, David W. G. Stanton, Stephen M. Thomas, A. Donald A'Bear, Jennifer Hiscox, T. Hefin Jones, Jana Voříšková, Petr Baldrian, Lynne Boddy. 2013. Top-down control of soil fungal community composition by a globally distributed keystone consumer. Ecology 94:2518–2528. http://dx.doi.org/10.1890/13-0197.1

Appendix A. Fluorogenic artificial substrates available commercially (MUF = 4-methylumbelliferone) for the enzyme activity measurements.

Table A1. Fluorogenic artificial substrates available commercially (MUF = 4-methylumbelliferone) for the enzyme activity measurements. Corresponding terminal groups are separated from macromolecules and from smaller soluble substrates.

Enzyme

Substrate

Element

Macromolecule degraded

Cellobiosidase (CBH), E.C 3.2.1.91

4-MUF-ß-cellobiopyranoside

Carbon

Cellulose

ß-Glucosidase (BG), E.C. 3.2.1.21

4-MUF-ß-D-glucopyranoside

Carbon

Cellulose

α-Glucosidase (AG), E.C. 3.2.1.20

4-MUF-α-D-glucopyranoside

Carbon

Starch and glycogen

ß-Xylosidase (BX), E.C. 3.2.1.37

4-MUF-ß-D-xylopyranoside

Carbon

Xylane, xylobiose

Chitinase (NAG), E.C. 3.2.1.30

4-MUF-N-acetyl-ß-D-glucosaminide

Carbon and Nitrogen

Breaking ß-1-4-glycosidic bonds in
N-acetyl-glucosaminide (chitin) and chitobiose

Phosphodiesterase (PDE), E.C. 3.1.4.1

bis-(4-MUF)-phosphate

Phosphorus

Hydrolysis of phosphate diesters

Phosphomonoesterase (AP), E.C. 3.2.1.30

4-MUF-phosphate

Phosphorus

Hydrolysis of phosphate diesters

Arylsulphatase (AS), E.C. 3.1.6.1

4-MUF-sulphate

Sulphur

Mineralization of organic sulphur


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