Ecological Archives E094-257-A1
Johannes R. Björk, C. Díez-Vives, Rafel Coma, Marta Ribes, José M. Montoya. 2013. Specificity and temporal dynamics of complex bacteria–sponge symbiotic interactions. Ecology 94:2781–2791. http://dx.doi.org/10.1890/13-0557.1
Appendix A. Figures showing different analyses conducted using denaturing gradient gel electrophoresis (DGGE) analysis and a dendrogram of the monthly Jaccard coefficient of similarity for the bacterioplankton.
Fig A1. DGGE analysis of 16S rRNA amplicons using 16S rDNA bacterial primers. (a) Monthly samples over one year of Agelas oroides with 4 replicates in March, and (b) the bacterioplankton with 4 replicates in October. Numbered dots mark the bands that were further excised and sequenced (see Table C1). (c) DGGE image showing the relative distribution of the bands for the three sponge hosts and the bacterioplankton.
Fig. A2. Dendrogram showing the monthly Jaccard coefficient of similarity for the bacterioplankton. October has 5 replicates, more similar between themselves than to any other month (i.e., the individual variability within months are low). Red branches indicate that SIMPROF analysis could not find any statistical evidence for any sub-structure within these clusters.
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