Ecological Archives E095-013-A3
Alexandra H. Campbell, Adriana Vergés, Peter D. Steinberg. 2014. Demographic consequences of disease in a habitat-forming seaweed and impacts on interactions between natural enemies. Ecology 95:142–152. http://dx.doi.org/10.1890/13-0213.1
Appendix C. Description of methodology for culturing bacteria used in inoculation experiment.
Cultures of Nautella sp. R11 were grown in 1 L of marine broth (Difco) liquid medium at a temperature of 30 °C on an orbital mixer set to 150 rpm to stationary phase, when cells reached a density of approximately 109 cells/mL (Case et al. 2011). Most laboratory-based inoculation experiments aimed at testing the efficacy of pathogens against hosts use a cell density of approximately 108 cells/mL (e.g., Cervino et al. 2008). We used a slightly higher inoculum density (109 cells/mL) for these field experiments to account for dilution within the dosage bags (see below), which were 3500 mL in volume, giving a final volume of approximately 107 cells/mL.
Cervino, J. M., F. L. Thompson, B. Gomez-Gil, E. A. Lorence, T. L. Goreau, R. L. Hayes, K. B. Winiarski-Cervino, Smith, G. W., Hughen, K., and E. Bartels. 2008. The Vibrio core group induces yellow band disease in Caribbean and Indo-Pacific reef-building corals. Journal of Applied Microbiology 105:1658–1671.
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